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EFFECTS OF OZONE EXPOSURE ON LIPID METABOLISM IN HUMAN ALVEOLAR MACROPHAGES
Citation:
Friedman, H., M. Madden, J. Samet, AND H. Koren. EFFECTS OF OZONE EXPOSURE ON LIPID METABOLISM IN HUMAN ALVEOLAR MACROPHAGES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-91/046.
Description:
Alveolar macrophages (AM) store arachidonic acid (AA) which is esterified in cellular phospholipids until liberated by phospholipase A2 or C after exposure to inflammatory stimuli. ollowing release, there can be subsequent metabolism of AA into various potent, biological active mediators including prostaglandins and platelet activating factor (PAF). o examine the possibility that these mediators may account for some of the pathophysiologic alterations seen in the lung following O3 exposure, human AM were collected by bronchoalveolar lavage of normal subjected into tissue culture dishes, and the adherent cells were incubated with 3H-AA or 3H-lysoPAF. uman AM exposed 1.0 ppm O3 for 2 hr released 65 + 12% more tritium, derived from 3H-AA, than paired air-exposed controls into media supernatants. n other studies using a similar O3 exposure protocol, there was also a significant increase in human AM PGE2 production (2.0 + 0.5 fold-increase above air-exposure values, p