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MULTIPLE REPLICONS CONSTITUTING THE GENOME OF PSEUDOMONAS CEPACIA 17616
Citation:
Cheng, H. AND T. Lessie. MULTIPLE REPLICONS CONSTITUTING THE GENOME OF PSEUDOMONAS CEPACIA 17616. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/446 (NTIS PB95112132).
Description:
Macrorestriction fragment analysis of DNA from Pseudomonas cepacia 17616, in conjunction with Southern hybridization experiments using junction n enzyme sites as probes, indicated that this bacterium contains three large circular replicons of 3.4, 2.5, and 0.9 170-kb cryptic plasmid present in this strain gave an overall estimate of genome size of 7 Mb. ther Southern that the three large replicons contained r RNA genes as well as insertion sequence elements identified previously in Pacl, and Pmel sites on the three replicons was determined. erivative of Tn5-751 carrying a Swal site was used to inactivate and map genes on the 2.5- and 3.4-Mb replicons. utants were isolated in which the 2.5- and 0.9-Mb replicons had been reduced in size to 1.8 and 0.65 Mb, respectively. he loss of DNA from the 2.5-Mb replicon was associated with lysine auxotrophy, B-lactonase deficiency, and failure to utilize ribitol and trehalose as carbon and energy sources. NA fragments corresponding in size to randomly linearized forms of the different replicons were detected in unrestricted DNA by pulsed-field gel electrophoresis. he results provide a framework for further genetic analysis of strain 17616 and for evaluation of the genomic complexities of other P. cepacia isolates.
