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Assessing Environmental Surface Water Exposure by Cell Culture-based Metabolomics (2019 ASMS)
Citation:
Yue, Y., J. Mosley, P. Bradley, J. Cavallin, D. Villeneuve, G. Ankley, D. Ekman, T. Collette, AND Q. Teng. Assessing Environmental Surface Water Exposure by Cell Culture-based Metabolomics (2019 ASMS). 2019 ASMS Conference on Mass Spectrometry and Allied Topics, Atlanta, GA, June 02 - 06, 2019.
Impact/Purpose:
In this study, we applied cell culture-based metabolomics to investigate impacted biological pathways by chemical contaminants in surface water samples collected from 8 stream sites. The cell-based assay results of environmental surface waters from eight sites indicated changes in site-specific biological pathways, which in general agreed with the total number and overall concentrations of detected chemicals.
Description:
The presence of anthropogenic contaminants in environmental surface waters often results in potential health risks for humans and wildlife. As promising approaches, ’omics techniques have been developed to assess environmental risk and evaluate potential toxic impacts of chemical contaminants. Metabolomics is the ‘omics approach to understanding cell and system level biology, and has been applied in vitro for monitoring biological impacts of water contaminants and aquatic environmental stressors. Combined with high-resolution mass spectrometry (HR MS), metabolomics can investigate endogenous metabolites of organisms and cells, and their significant variations under chemical exposure for discovering potential biomarkers, elucidating metabolic pathway alternations, and assessing biological impacts of chemical exposure. In this study, we applied cell culture-based metabolomics to investigate biological pathways impacted by complex mixtures of chemical contaminants in surface water samples collected from eight different streams. These streams were part a larger nationwide study and chosen based on their representation of different land uses and anticipated chemical contaminant profiles. Two human cell lines (liver cell; HepG2 and brain cell; LN229) and one fish cell line (zebrafish liver; ZFL) were exposed for 48 hours to water samples from each stream. HR LC-MS was applied to measure changes (relative to controls) of endogenous polar metabolites. These changes were then used to determine the primary biological pathways that were impacted, and thus potential adverse impacts in exposed biota.