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GENUS- AND GROUP-SPECIFIC HYBRIDIZATION PROBES FOR DETERMINATIVE AND ENVIRONMENTAL STUDIES OF SULFATE-REDUCING BACTERIA
Citation:
Devereux, R., M. Kane, J. Winfrey, AND D. Stahl. GENUS- AND GROUP-SPECIFIC HYBRIDIZATION PROBES FOR DETERMINATIVE AND ENVIRONMENTAL STUDIES OF SULFATE-REDUCING BACTERIA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/064.
Description:
A set of six oligonucleotides, complementary to conserved tracts of 16S rRNA from phylogenetically-defined groups of sulfate-reducing bacteria, was characterized for use as hybridization probes in determinative and environmental microbiology. our probes were genus specific and identified Desulfobacterium spp., Desulfobacter spp., Desulfobulbus spp., or Desulfovibrio spp. he other two probes encompassed more diverse assemblages. ne probe was specific for the phylogenetic lineage composed of Desulfococcus multivorans, Desulfosarcina variabilis, and Desulfobotulus sapovorans. he remaining probe was specific for Desulfobacterium spp., Desulfobacter spp., D. multivorans, D. variabills, and D. sapovorans. emperature of dissociation was determined for each probe and the designed specificities of each were evaluated by hybridizations against closely related nontargeted species. n addition, each probe was screened by using a "phylogrid" membrane which consisted of nucleic acids from sbctyfour non-targeted organisms representing a diverse collection of eukarya, archaes, and bacteria. he value of these probes to studies in environmental microbiology was evaluated by hybridizations to 16S rRNAs of sulfate-reducing bacteria present in marine sediments.