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PRELIMINARY CULTURE AND LIFE-CYCLE EXPERIMENTS WITH THE BENTHIC AMPHIPOD AMPELISCA ABDITA
Citation:
Redmond, M., K. Scott, R. Swartz, AND J. Jones. PRELIMINARY CULTURE AND LIFE-CYCLE EXPERIMENTS WITH THE BENTHIC AMPHIPOD AMPELISCA ABDITA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/566.
Description:
The tube-dwelling amphipod Ampelisca abdita Mills 1964 has been used extensively in acute sediment toxicity tests and has been shown to be amenable to chronic testing. mpelisca abdita was held in the lab through several generations when fed algal food in daily static renewals, although culturing success was not consistent. lgal food consisted of one or more of the following: the flagellate Pseudoisochrysis paradoxa Sutton, and the diatoms Phaeodactylum tricornutum Bohlin and Chaetoceros calcitrans (Paulsen) Tokano. ensitivity of cultured animals to cadmium chloride in 96-h seawater-only tests (LC50s 0.28-0.58 mg Cd/L) was comparable to that of field-collected animals (LC50 0.20 mg Cd/L). ife-cycle test initiated with juveniles 8 to 10 d old resulted in production of young or fertilized broods in only two of the 12 sample containers in which young were expected. mphipods were sexually mature at approximately 20 d of age at 25 degrees C, and young were first produced at 34 to 36 d. Short-term tests were used to quantify growth of this species in 10 to 14 d. Results from a variety of experiments indicated that there are still one or more unresolved problems with the culture and chronic testing of Ampelisca abdita. actors such as nutrition. flow rate, light, and temperature need to bc examined further.