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DNA ADDUCTS AND INDUCTION OF SISTER CHROMATID EXCHANGES IN THE RAT FOLLOWING BENZO(B)FLUORANTHENE ADMINISTRATION
Citation:
Ross, J., G. Nelson, K. Holden, A. Kligerman, G. Erexson, M. Bryant, K. Earley, A. Beach, R. Gupta, AND S. Newnow. DNA ADDUCTS AND INDUCTION OF SISTER CHROMATID EXCHANGES IN THE RAT FOLLOWING BENZO(B)FLUORANTHENE ADMINISTRATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/438.
Description:
Benzo[b]fluoranthene (B[b]F) was administered (100 mg/kg by i.p. injection) to male Sprague-Dawley rats. Lungs, livers, and peripheral blood lymphocytes (PBLs) were harvested 1, 3, 5, 7, 14, 28, and 56 days after treatment. Several DNA adducts were observed in each tissue, with maximal levels occurring at about 7 days after treatment. Lung DNA exhibited consistently higher adduct levels than liver or PBL DNA. At 56 days the adducts in liver and PBL DNA were present at less than 10 amoles/ug DNA, but in lung there were still 100 amoles/ug DNA. No significant differences were observed between tissues in the types of adducts produced. Co-chromatography with synthetic standards showed that only a minor adduct produced in is derived from benzo[b]fluoranthene-trans-9,10-dihydrodiol-11,12-epoxide. ister chromatid exchanges (SCEs) from whole blood cultures were significantly increased relative to concurrent controls between 1 and 14 days after B[b]F administration, with maximum levels at 14 days. However, SCE induction did not correlate with the amount of B[b]F-DNA adducts remaining in the PBLs at harvest time. By 28 days after treatment, SCEs had essentially returned to control levels.