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Modeling the Neurovascular Unit In Vitro and In Silico
Citation:
Saili, K., T. Zurlinden, AND T. Knudsen. Modeling the Neurovascular Unit In Vitro and In Silico. Chapter 11, Handbook of Developmental Neurotoxicology’, 2nd edition. Academic Press Incorporated, Orlando, FL, , 127-142, (2018). https://doi.org/10.1016/B978-0-12-809405-1.00011-0
Impact/Purpose:
Chemical interactions with the developing neurovascular unit (NVU) and the blood-brain barrier (BBB) are an important, yet understudied aspect of developmental neurotoxicology. This chapter focuses on in vitro and in silico models of BBB development as a basis for understanding and predicting developmental neurotoxicity.
Description:
Chemical interactions with the developing neurovascular unit (NVU) and the blood-brain barrier (BBB) are an important, yet understudied aspect of developmental neurotoxicology. Various in vitro and in silico models established for assessing human BBB function have not been assessed for their potential utility in predictive developmental neurotoxicity (DNT). Ideally, such in vitro models would derive from diverse human cell types comprising the NVU. Perfusable organoids and microphysiological systems that recapitulate functional vascularization have the potential to enable real-time data acquisition of molecular, biochemical, and cellular responses during chemical exposure. By coupling these platforms with computational (in silico) models that integrate variables of a complex system, predictive signatures of BBB disruption can be assimilated from high-throughput and high-content data streams. An integration of technologies to create a ‘virtual embryo’ has clear and present application to modeling neuronal organization during normal and induced abnormal development. This chapter focuses on in vitro and in silico models of BBB development for predictive developmental neurotoxicity.
