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EFFECT OF NONGENOTOXIC ENVIRONMENTAL CONTAMINATION ON CHOLESTEROL AND DNA SYNTHESIS IN CULTURED PRIMARY RAT HEPATOCYTES
Citation:
Reddy, T., L. Chang, A.B. DeAngelo, M. Pereira, AND F. Daniel. EFFECT OF NONGENOTOXIC ENVIRONMENTAL CONTAMINATION ON CHOLESTEROL AND DNA SYNTHESIS IN CULTURED PRIMARY RAT HEPATOCYTES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/396 (NTIS PB93236339).
Description:
The effect of certain reputedly non genotoxic agents on cholesterol and DNA synthesis was investigated in cultured rat primary hepatocytes and liver slices. epatocytes in culture were incubated for 48, 60, and 72 hrs with one of the following chemicals; namely, chloroform (CHCl3), 1,1,2-trichloroethane (TCE) 100 um; trichloroacetic acid (TCA), dichloroacetic acid (DCA) (1mM); monochloroacetic acid (MCA) (01. um); di(2-ethylhexyl) phthalate (DEHP), mono(ethylhexyl) phthalate (MEHP), 200 um, phenobarbital (PB) (1.5mM); epidermal growth factor (EGF) (100 ng/ml) dimethylsulfoxide (DMSO) (0.1%) stimulated cholesterol and DNA synthesis in the cultures. owever, the induction of DNA synthesis in hepatocytes treated with DCA became apparent by sixty hours.