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MICRONUCLEI IN BINUCLEATED LYMPHOCYTES OF MICE FOLLOWING EXPOSURE TO GAMMA RADIATION
Citation:
Erexson, G., A. Kligerman, E. Halperin, G. Honore, AND J.W. Allen. MICRONUCLEI IN BINUCLEATED LYMPHOCYTES OF MICE FOLLOWING EXPOSURE TO GAMMA RADIATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/232 (NTIS PB90145988).
Description:
Experiments were designed to investigate the induction of micronuclei (MN) in mouse peripheral blood lymphocytes (PBLs) after in vitro or in vivo exposure to 60Co gamma radiation. or the in vitro experiments, 4 ml of blood from male C57BL/6J mice were either irradiated in 6 ml Falcon culture tubes as whole blood or isolated to obtain mononuclear leucocytes (MNLs) that were pelleted by centrifugation and then irradiated in RPMl 1640. or the in vivo analysis mice received whole body irradiation, blood was obtained by cardiac puncture, and the MNLs were isolated for each mouse. Exposures were at a rate of 0.82 to 0.90 Gy/min to yield doses of 0.5, 1, 2, 3, or 4 Gy. NLs were cultured using cytochalasin B for MN analysis in binucleated PBLS. There was a significant dose-dependent increase in MN observed at all doses. ose-response curves for the in vivo and in vitro whole blood experiment were not significantly different. owever, for isolated pelleted MNLs irradiated in vitro, the frequency at 4 Gy was less than half that seen in the in vivo experiment. The large difference in MN response is thought to be due to the radioprotective effect of hypoxia.
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MICRONUCLEI IN BINUCLEATED LYMPHOCYTES OF MICE FOLLOWING EXPOSURE TO GAMMA RADIATION