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SPERMATID MICRONUCLEUS ANALYSES OF TRICHLOROETHYLENE AND CHLORAL HYDRATE EFFECTS IN MICE
Citation:
Allen, J.W., B. Collins, AND P. Evansky. SPERMATID MICRONUCLEUS ANALYSES OF TRICHLOROETHYLENE AND CHLORAL HYDRATE EFFECTS IN MICE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/316 (NTIS PB94197209).
Description:
Mice were exposed by inhalation to trichloroethylene (TCE), or by i.p. injection to the TCE metabolite, chloral hydrate (CH). arly spermatids were analyzed for micronucleus (MN) frequency and kinetochore status (presence or absence) using fluorochrome-labeled anti-kinetochore antibodies. t was determined that 5 consecutive days of exposure to S, 50 or 500 ppm TCE during preleptotene through early pachytene stages of meiotic cell development does not result in increased frequencies of spermatid MN. H at 41, 83 or 165 mg/kg was positive for spermatid MN induction when treatments corresponded to spermatogonial stem cell or preleptotene spermatocyte stages of development; negative results were obtained after treatments of leptotene-zygotene or diakinesis-metaphase stages. he significantly increased levels of MN observed were invariably of the kinetochore-negative type.