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CYTOTOXICITY OF CHEMICAL CARCINOGENS TOWARDS HUMAN BRONCHIAL EPITHELIAL CELLS EVALUATED IN A CLONAL ASSAY
Citation:
Siegfried, J. AND S. Nesnow. CYTOTOXICITY OF CHEMICAL CARCINOGENS TOWARDS HUMAN BRONCHIAL EPITHELIAL CELLS EVALUATED IN A CLONAL ASSAY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-84/412.
Description:
Survival of human bronchial epithelial cells after administration of four chemical carcinogens was measured in a clonal assay. Human bronchial epithelial cells were obtained from outgrowths of explanted tissue pieces. Serum-free medium was used for both explant culture and clonal growth. The clonal assay could be performed on three substrata: plastic dishes alone, protein-coated dishes, and inactivated Swiss 3T3 cells. Several other cell lines supported clonal growth of the human cells. Little variation among individuals in cytotoxicity of N-methyl-N'-nitro-N-nitrosoguanidine and K2CrO4, a possible human lung carcinogen, was observed, but in the case of benzo(a)pyrene and 7,12-dimethylbenz(a)anthracene, large variations in survival were found between cultures derived from different individuals. (Copyright (c) IRL Press Ltd.)