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COMPARATIVE IN VITRO PERCUTANEOUS ABSORPTION OF P-SUBSTITUTED PHENOLS THROUGH RAT SKIN USING STATIC AND FLOW-THROUGH DIFFUSION SYSTEMS
Citation:
Hughes, M., S. Shrivastava, H. Fisher, AND L. Hall. COMPARATIVE IN VITRO PERCUTANEOUS ABSORPTION OF P-SUBSTITUTED PHENOLS THROUGH RAT SKIN USING STATIC AND FLOW-THROUGH DIFFUSION SYSTEMS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/416.
Description:
The objective of this study was to determine the in vitro percutaneous absorption of [14C]-phenol and eight p-substituted derivatives and to examine the variability of this data. wo diffusion systems, the static and flow-through, were used. lipped dorsal skin was removed from female F344 rats (90 days old), dermatomed to a thickness of approximately 350 um, placed in a diffusion cell and treated with chemical (4 ug/cm2, in ethanol). he cumulative absorption at 72 hr for the phenols in both systems ranged from 5.3 to 97.6%. Greater than 70% of the chemical was absorbed in both systems when the chemical had a log P value between 1.4-3.5. Significant differences in percent absorption between the two diffusion systems was observed with 5 of the compounds. bsorption of acetamidophenol, chlorophenol and cyanophenol were significantly lower in the static system. bsorption of phenol and heptyloxyphenol were significantly lower in the flow-through system. he most variable absorption data in both systems were with the phenols that were absorbed the least ( 70%). hese same chemicals were also on the extremes of the log P scale for the phenols examined. ontrolling for the variability of in vitro percutaneous absorption of chemicals is necessary when using the data for human risk assessment.