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PALATAL EXPRESSION OF TGFB ISOFORMS IN NORMAL AND RETINOIC ACID-TREATED EMBRYOS
Citation:
Fitzpatrick, D., B. Abbot, AND R. Akhurst. PALATAL EXPRESSION OF TGFB ISOFORMS IN NORMAL AND RETINOIC ACID-TREATED EMBRYOS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-91/127 (NTIS PB91213595).
Description:
Retinoic Acid (RA) is know to induce cleft palate in all mammalian species tested. he aetiology of RA-induced cleft palate has been extensively investigated in C57B16 mouse embryos by one of us 1. e have recently shown distinct site- and stage-specific expression pattern of the RNAs encoding the three TGFB isoforms (TGFB1, TGFB2 and TGFB3) during normal murine palatogenesis suggesting an important role for these regulators of cell function in this developmental process. GFB1 and TGFB3 are expressed in the medial edge epithelium at the time of palatal fusion, whereas TGFB2 RNA is exclusively mesenchymal. n the present study we have investigated the expression of TGFBs in mouse embryo palates from mothers treated in vivo with 100mg/kg all-trans RA on day 10 post coitum (p.c.) and collected on day 12 and day 14 p.c. he RA-treated embryos showed marked hypoplasia of the palatal shelves but, surprisingly, did not show any major alteration In the RNA localization of TGFB1 or TGFB3. here were, however, minor changes in the expression characteristics of TGFB2 RNA.
