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DETERMINATION OF PENTACHLOROPHENOL IN MARINE BIOTA AND SEA WATER BY GAS-LIQUID CHROMATOGRAPHY AND HIGH-PRESSURE LIQUID CHROMATOGRAPHY
Citation:
Faas, L. AND J. Moore. DETERMINATION OF PENTACHLOROPHENOL IN MARINE BIOTA AND SEA WATER BY GAS-LIQUID CHROMATOGRAPHY AND HIGH-PRESSURE LIQUID CHROMATOGRAPHY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-79/053 (NTIS PB80163678).
Description:
A method is described for measuring pentachlorophenol (PCP) in samples from the estuarine environment. Gas-liquid chromatography (GLC) is used to determine PCP residues in tissues as low as 0.01 ppm by formation of the ethyl diazohydrocarbon derivative, followed by Florisil cleanup. Application of the method to exposed organisms indicates that PCP accumulates in mullet (Mugil cephalus), grass shrimp (Palaemonetes pugio), and eastern oysters (Crassostrea virginica). Sea water concentrations as low as 0.002 ppb may be detected by formation of the amyl diazohydrocarbon derivative. Formation of the amyl derivatives of PCP and several related compounds gives GLC separation not possible with the methyl or ethyl derivatives. Parameters are outlined for high-pressure liquid chromatography (LC) determination of the free phenol without cleanup. Ultraviolet detection limits for PCP by LC are 5.0 ppm in tissues and 2.0 ppb in seawater.