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EFFECTS OF ETHANOL, PHENOL, FORMALDEHYDE, AND SELECTED METABOLITES ON METABOLIC COOPERATION BETWEEN CHINESE HAMSTER V79 LUNG FIBROBLASTS
Citation:
Malcolm, A., L.J. Mills, AND J. Trosko. EFFECTS OF ETHANOL, PHENOL, FORMALDEHYDE, AND SELECTED METABOLITES ON METABOLIC COOPERATION BETWEEN CHINESE HAMSTER V79 LUNG FIBROBLASTS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-85/119 (NTIS PB85220390).
Description:
The discovery that phorbol ester tumor promoters inhibit metabolic cooperation between cultured cells in proportion to their promoting activity in vivo suggests that such inhibition may be a mechanism in tumor promotion. Because metabolic cooperation appears to be essential for normal cell differentiation and tissue development, the concept that chemicals might be toxic by blocking this process may, in theory, be extended to reproductive system toxicants. In the paper, these hypotheses are tested by adding test chemical and selective agent (6-thioguanine) simultaneously to co-cultivated mutant(HGPRT-) and wild-type(HGPRT+) V79 cells in reconstructed mutant selection experiments. Effects of test chemicals are measured as a function of mutant cell recovery relative to that in a control.