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PHYSICAL AND CHEMICAL CONTROL OF RELEASED MICROORGANISMS AT FIELD SITES
Citation:
Donegan, K., R. Seidler, AND C. Matyac. PHYSICAL AND CHEMICAL CONTROL OF RELEASED MICROORGANISMS AT FIELD SITES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/080 (NTIS PB92153360).
Description:
An important consideration in the environmental release of a genetically engineered microorganism (GEM) is the capability for reduction or elimination of GEM populations once their function is completed or if adverse environmental effects are observed. In this study the decontamination treatments of burning and biocide application, alone and in combination with tilling, were evaluated for their ability to reduce populations of bacteria released on the phylloplane. ield plots of bush beans sprayed with the bacterium Erwinia herbicola, received the following treatments: 1. control, 2. control + till, 3. burn, 4. burn + till, 5. Kocide (cupric hydroxide), 6. Kocide + till, 7. Agri-strep (streptomycin sulfate), and 8. Agri-strept + till. eaves and soil from the plots were sampled -1,1,5,8,12,15, 19, and 27 days after application of the decontamination treatments. urning produced a significant and persistent reduction in the number of bacteria whereas tilling, alone or in combination with the biocide treatments, stimulated a significant and persistent reduction in the number of bacteria whereas tilling, alone or in combination with the biocide treatments, stimulated a significant increase in bacterial populations that persisted for several weeks. he individual treatments of the biocides, Kocide and Agri-strep, produced a decline in bacterial populations which did not significantly differ from that of the control treatment.
