You are here:
ENHANCEMENT OF BIODEGRADATION OF ALASKAN WEATHERED CRUDE OIL COMPONENTS BY INDIGENOUS MICROBIOTA WITH THE USE OF FERTILIZERS AND NUTRIENTS
Citation:
Tabak, H., A. Venosa, J. Glaser, J. Haines, S. Desai, AND W. Nisamaneepong. ENHANCEMENT OF BIODEGRADATION OF ALASKAN WEATHERED CRUDE OIL COMPONENTS BY INDIGENOUS MICROBIOTA WITH THE USE OF FERTILIZERS AND NUTRIENTS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/A-93/224 (NTIS PB93236537).
Description:
Bench-scale biodegradability studies of the Alaskan weathered crude oil were undertaken as part of the bioremediation project for the shorelines of Prince William Sound, Alaska, contaminated by the Exxon oil spill. he purpose of the studies was to evaluate the capability of the indigenous microbial consortium of the sea water and island beach areas, to biodegrade the weathered crude oil alkane hydrocarbon and polynuclear aromatic (PAH) constituents in batch-type respirometric reactors and shaker flask systems. iodegradation studies incorporated the use of INIPOL EAP22 and water soluble nitrogen and phosphorus containing solution (OECD) as the fertilizer nutrient source. n the respirometric studies, the unpolluted beach material in seawater was spiked with 1,000,300 and 100 mg/L of weathered crude oil and with INIPOL at 5% of oil weight or with OECD stock solution concentrates. he contents were brought up to 1,000 mL with seawater. npolluted beach material spiked with 10,000, 3,000, and 1,000 mg/L of weathered crude oil and with either INIPOL at 5% of oil weight or with OECD inorganic salts in 1,000 mL of seawater were used in shakerflask microcosm studies. emperature of incubation in both studies was 15 degrees C, and culture systems were incubated in darkness.