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DETECTION AND INACTIVATION OF ENTERIC VIRUSES IN WASTEWATER
Citation:
Shuval, H. AND E. Katzenelson. DETECTION AND INACTIVATION OF ENTERIC VIRUSES IN WASTEWATER. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/2-77/095 (NTIS PB270210), 1977.
Description:
This report covers studies on the development and evaluation of methods for concentrating and assaying low levels of viruses in large volumes of water as well as studies on the use of ozone in inactivating viruses in water and wastewater. Of the eight virus concentration methods evaluated, filtration with cellulose nitrate membranes, aluminum hydroxide and PE-60 proved most promising. The feasibility of using hollow fiber membranes was demonstrated. A rapid method capable of detecting viruses in water in less than 24 hours using fluorescent antibodies was developed. A spectrophotometric method of detecting low concentrations of ozone in small (10 ml) samples of water was developed. Kinetic studies show that ozone inactivates enteroviruses more rapidly than chlorine under comparable conditions. With a 0.3 ppm residual ozone inactivates 99% of seeded poliovirus in clean water in less than 10 seconds as compared to 100 seconds for chlorine.