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Fish oil and olive oil-rich diets modify ozone-induced cardiovascular effect in rats
Citation:
Tong, H., S. Snow, M. Schladweiler, AND U. Kodavanti. Fish oil and olive oil-rich diets modify ozone-induced cardiovascular effect in rats. Society of Toxicology Annual Meeting, Baltimore, MD, March 12 - 16, 2017.
Impact/Purpose:
This study examined the cardiovascular effects of ozone and the efficacy of dietary supplements in attenuating these effects of ozone exposure.
Description:
Rationale: Air pollution exposure has been associated with adverse cardiovascular health effects. Our clinical studies suggest that fish oil (FO) and olive oil (OO) supplementations attenuate the cardiovascular responses to inhaled concentrated ambient particles. This study was designed to examine the cardiovascular effects of ozone and the efficacy of FO and OO-rich diets in attenuating these effects of ozone exposure in rats. Methods: Male Wistar Kyoto rats were fed either a normal diet (ND), or a diet enriched with 6% FO or OO starting at 4 weeks of age. Eight weeks following the start of the diet, animals were exposed to filtered air (FA) or 0.8 ppm ozone, 4 hr/day for 2 consecutive days. Immediately after exposure, cardiac responses were assessed ex vivo using a Langendorff heart preparation with a protocol consisting of 20 min of global ischemia followed by 2 hr reperfusion. Cardiac function was measured as the index of left-ventricular developed pressure (LVDP) and contractility (dP/dtmax and dP/dtmin) before ischemia. Upon reperfusion after ischemia, the recovery of post-ischemic LVDP and infarct size were examined. Results: The pre-ischemic LVDP, dP/dtmax, and dP/dtmin were lower after ozone exposure when compared to the FA control in the rats fed ND but not FO and OO. OO diet shortened the time to ischemic contracture of the hearts after FA exposure compared to ND. Ozone exposure increased pre-ischemic heart rate and the time to ischemic contracture compared to the FA control in rats fed OO. However, there were no significant differences in the post-ischemic recovery of cardiac function and the infarct size among the exposure or diet groups. Conclusions: This study demonstrated that ozone-induced cardiovascular responses appear to be blunted by FO and OO diets. (This abstract does not necessarily reflect EPA policy.)