Citation:

Moser, V. AND W. Boyes. PROLONGER NEUROBEHAVIORAL AND VISUAL EFFECTS OF SHORT-TERM EXPOSURE TO 3,3'-IMINODIPROPIONITRILE (IDPN) IN RATS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/069 (NTIS PB94141579).

Description:

Strategies for neurotoxicity testing often include initial screening tests, such as a functional observational battery (FOB) and motor activity assessment, followed by detailed characterization studies. n this study, a neurobehavioral screening battery (FOB and motor activity) was used to evaluate the effects of three-day repeated exposure to either 0, 100, 200, or 400 mg/kg/day IDPN. dult Long-Evans rats (males and females) were tested before dosing, and 1, 14, 28, 56, and 91 days after the third dose. DPN initially produced generalized CNS depression, weakness, and hypothermia. hereafter, marked hyperactivity, increased excitability, decreased reactivity to visual and auditory stimuli, neuromuscular weakness, equilibrium changes, and a "waltzing syndrome" vertical and lateral head movements, circling, and retropulsion emerged and persisted for three months. ales were more severely affected than females. ollowing neurobehavioral testing, the rats were examined for visual function using flash- (three intensities) and pattern- (three pattern sizes by three contrast levels) elicited visual evoked potentials (VEPs). DPN produced changes in pattern- and flash-elicited VEPs, thus verifying predictions made from the screening tests. owever, the extent of the VEP changes produced by IDPN was insufficient to account for some of the deficits detected in the FOB, which are dependent on sensory, integrative, and motor functions. hus, profound neurological effects of IDPN were detected using this screening battery and visual effects were confirmed using VEPS. hese effects, following only three doses of IDPN, lasted for at least three months and thus may be permanent.

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