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MUTATION SPECTRA IN SALMONELLA OF SUNLIGHT, WHITE FLUORESCENT LIGHT, AND LIGHT FROM TANNING SALON BEDS: INDUCTION OF TANDEM MUTATIONS AND ROLE OF DNA REPAIR
Citation:
DeMarini, D.M., M. Shelton, AND L. Stankowski, Jr. MUTATION SPECTRA IN SALMONELLA OF SUNLIGHT, WHITE FLUORESCENT LIGHT, AND LIGHT FROM TANNING SALON BEDS: INDUCTION OF TANDEM MUTATIONS AND ROLE OF DNA REPAIR. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/296.
Description:
We evaluated the mutagenicity of sunlight (SUN), uncovered cool white fluorescent light (FLR), and light from a tanning salon bed (TAN) at the base-substitution allele hisG46 of Salmonella in four DNA repair backgrounds (wild type, uvrB, pKM101, and uvrB+pKM101). Approximately 80% of the radiation emitted by TAN was within the ultraviolet (UV) range whereas only 10% of the SUN and 1% of FLR radiation was UV. AN emitted similar amounts of UVA and UVB, whereas SUN emitted 50-60X and FLR emitted 5-10X more UVA relative to UVB. ased on total dose (UV + Visible), the mutagenic potency ranking was TAN greater than FLR greater than SUN. he uvrB mutation enhanced the mutagenicity of the environmental UV sources more (20-216X) than did the pKM101 plasmid (20X) relative to wild-type DNA repair. he majority of the mutations were GC to AT transitions. ll light source; induced equal proportions of transitions and transversions in excision repair-profile strains, but they induced more transitions relative to transversions in uvrB-containing strains. ultiple mutations, which were the only mutational class enhanced by both uvrB and pKM101 either alone or together for all three light sources, accounted for 3-5% of the induced mutations in the plasmid-containing strains. he increases in multiple mutations in TA100 (uvrB, pKM101) were the highest of any mutational class (38-82 fold). f the TAN-induced mutations, 83% (19/23) were CC to TT tandem transitions. hese results show for the first time that FLR and TAN produced mutations similar to those produced by SUN, a known carcinogen for human skin cancer.