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OZONE EFFECT ON RESPIRATORY SYNCYTIAL VIRUS INFECTIVITY AND CYTOKINE PRODUCTION BY HUMAN ALVEOLAR MACROPHAGES
Citation:
Soukup, J., H. Koren, AND S. Becker. OZONE EFFECT ON RESPIRATORY SYNCYTIAL VIRUS INFECTIVITY AND CYTOKINE PRODUCTION BY HUMAN ALVEOLAR MACROPHAGES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/215 (NTIS PB94163946).
Description:
This study was performed to evaluate the effect of ozone (O3) exposure at 1 ppm for 2 hours, on the susceptibility/resistance of adult human alveolar macrophages (AM) to infection with respiratory syncytial virus (RSV) in vitro, and RSV-induced cytokine production by the AM. M were first exposed to O3 or to filtered air and then infected with RSV at multiplicities of infection (moi) 0.1, 1.0 and 10. he percent RSV infected AM and the amount of infectious virus released by the cells were determined at days 2 and 4 after infection. IL-1, IL-6, and TNF levels in the supernatants were determined on day 2. No difference in the percent infected AM or in the amount of infectious RSV produced were found between control and O3-exposed cultures. owever, O3-exposed AM infected with RSV at moi 1 produced less IL-1 in response to RSV infection that control AM; 63.6 pg/ml compared to 98.5 pg/ml. o difference in IL-1 was seen with moi 10. L-6 levels were also decreased, but only after infection with moi 0.1. At this level of infection, 830 pg/ml were produced by control-exposed AM as compared to 468.2 pg/ml by O3-exposed AM. TNF production was-unaffected by O3 at all moi. tatistical analysis of the O3 effect on AM cytokine production induced by the different moi, however, revealed no significant effect of 01. ased on these,observations, it appears unlikely that O3, alters susceptibility of AM to infection with-RSV, nor does O3 dramatically alter cytokine production in response to RSV, since effects on IL-1 and IL-6 secretion were only found with the lowest levels of infection which induced cytokine release.