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ROLE OF STEROID HORMONES AND DECIDUAL INDUCTION IN THE REGULATION OF ADENOSINE DIPHOSPHORIBOSYL TRANSFERASE ACTIVITY IN RAT ENDOMETRIUM
Citation:
Cummings, A. ROLE OF STEROID HORMONES AND DECIDUAL INDUCTION IN THE REGULATION OF ADENOSINE DIPHOSPHORIBOSYL TRANSFERASE ACTIVITY IN RAT ENDOMETRIUM. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/210 (NTIS PB90146218), 1989.
Description:
To assess the effect of ovarian steroid hormones on enzyme activity, adenosine diphosphoribosyl transferase (ADPRT) was measured in endometrial nuclei isolated on estrus and on d 4 from rats ovariectomized on estrus (d 0) and treated d 0-3 with (a) vehicle, (b) 1 ug estrone/d (E), (c) l mg progesterone on d 0 and 2 mg d 1-3 (P), or (d) the 2 hormones combined (E+P). Decidual induction in rats treated with E+P through d 8 demonstrated the ability of the combination treatment to support a decidual response comparable to that of pseudopregnancy. Characteristics of ADPRT were assessed by the determination of assay optima, and the identity of the reaction product as poly(ADP-ribose) was confirmed. Deoxyribonuclease-stimulated activity (total) was higher than non-stimulated ADPBT activity (endogenous) in all assays except on estrus. Estrone treatment produced an increase in ADPRT activity accompanied by increased DNA content, tissue mass, and total protein. When compared with vehicle-treated controls, P alone did not affect ADPBT activity or DNA content, while E+P increased only ADPHT activity. Enzyme activity and DNA content following combination treatment were significantly lower than following estrone treatment, while tissue mass and protein content remained at the E-stimulated levels. he parallel stimulation of ADPRT activity and NA synthesis by E may reflect a previousLy suggested role for ADPRT in cellular proliferation. The blockade of E-stimulated ADPRT activity by P is considered a specific phenomenon which may be involved in the pattern of endometrial metabolism occurring during preimplantation differentiation.