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COMPETITION POTENTIALS OF ENVIRONMENTALLY APPLIED BACTERIA WITH HUMAN FECAL MICROBIOTA
Citation:
Nelson, G., L. Claxton, J. Creason, AND S. George. COMPETITION POTENTIALS OF ENVIRONMENTALLY APPLIED BACTERIA WITH HUMAN FECAL MICROBIOTA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/126 (NTIS PB93181147), 1992.
Description:
One of the potential human health effects associated with the environmental release. his of genetically engineered microorganisms is colonization of the intestinal tract study uses serial transfer techniques to monitor the in vitro survival and competition with human fecal microbiota of an environmentally released P. aeruginosa strain (BC16) isolated from a commercial product for polychlorinated biphenyl degradation. omparison of these results with previously obtained rodent in vivo and in vitro results can form a basis for extrapolating the potential for human health effects. uman and mouse fecal microbiota cultured in vitro were found to be nearly identical with respect to the numbers of various pollution enumerated on selective media. he .monitored populations included the total anaerobes, the obligately anaerobic Gram negative rods, the Gram positive anaerobes, the total aerobes, and the lactose-fermenting Enterobacteriaceae. n vitro survival of strain BC16 was also very similar in both the mouse and human system. train BC16 declined in number with each serial transfer until it was no longer detectable in culture 5. However, low numbers of BC16 were detected in serial transfer culture 5 for one human individual in one experiment. o alteration of the microbiota populations due to the addition of strain BC16 were found for either species. he comparable in vitro results for strain BC16 with human and mouse flora suggest that human and m us in vivo results might also be comparable. uture testing of additional strains is needed to establish a clear relationship between in vivo/vitro.