You are here:
DNA ISOLATION FROM SMALL TISSUE SAMPLES USING SALT AND SPERMINE
Citation:
Ross, J., G. Nelson, AND K. Holden. DNA ISOLATION FROM SMALL TISSUE SAMPLES USING SALT AND SPERMINE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/115 (NTIS PB93181048), 1991.
Description:
Common DNA isolation methods rely upon protein denaturation by organic solvents such as phenol and chloroform. hese solvents pose some risk to the user and require special disposal procedures. e have previously reported a method for isolating DNA from peripheral blood lymphocytes by precipitation with spermine. his procedure was less effective with other tissues, especially lung, and the spermine precipitates often took several days to dissolve in buffer. e have modified our procedure to include a pre-extraction of proteins ith concentrated NaCl, essentially as described by Miller et al. his current, improved procedure provides good recovery of purified DNA even ith small samples (<200 mg) and a variety of tissue and cell types.