You are here:
EFFECTS OF PHYSICOCHEMICAL FACTORS AND BACTERIAL COLONY MORPHOTYPE ON ASSOCIATION OF VIBRIO VULNIFICUS WITH HEMOCYTES OF CRASSOSTREA VIRGINICA
Citation:
Harris-Young, L., M. Tamplin, AND W. Fisher. EFFECTS OF PHYSICOCHEMICAL FACTORS AND BACTERIAL COLONY MORPHOTYPE ON ASSOCIATION OF VIBRIO VULNIFICUS WITH HEMOCYTES OF CRASSOSTREA VIRGINICA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/012 (NTIS PB94140688), 1993.
Description:
Vibrio vulnificus is a naturally occurring marine bacterium which causes invasive disease of immunocompromised humans following consumption of raw oysters. t is natural flora of Gulf Coast estuaries and has been found to inhabit tissues of oysters, Crassostrea virginica (Gmelin, 1791). he interaction of V. vulnificus with oyster host defenses has not been reported in detail. n the present studies, we examined the interaction of V. vulnificus with phagocytic oyster hemocytes in response to time, temperature, bacterial concentration, pretreatment In hemolymph, and with V. vulnificus translucent and opaque colonial morphotypes. ithin these experimental parameters, results showed that association of V. ulnificus with hemocytes increased with time, temperature, and V. vulnificus: hemocyte ratio. retreatment of V. vulnificus with serum, or increased serum concentration, did not enhance V. vulnificus hemocyte associations, indicating undetectable opsonic activity. ore than 50% of hemocytes bound the translucent, avirulent morphotype, whereas 10% to 20% were associated with the opaque, virulent form, indicating that degree of encapsulation was related to resistance to phagocytosis as previously described for mammalian phagocytes. nderstanding these cellular interactions may, in pan, explain persistence of V. vulnificus in oyster tissues and its ecology in estuarine environments.
URLs/Downloads:
EFFECTS OF PHYSICOCHEMICAL FACTORS AND BACTERIAL COLONY MORPHOTYPE ON ASSOCIATION OF VIBRIO VULNIFICUS WITH HEMOCYTES OF CRASSOSTREA VIRGINICA