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VALIDATION OF CHEMICAL AND BIOLOGICAL TECHNIQUES FOR EVALUATION OF VAPORS IN AMBIENT AIR/MUTAGENICITY TESTING OF TWELVE (12) VAPOR-PHASE COMPOUNDS
Citation:
Hughes, T., C. Sparacino, AND S. Frazier. VALIDATION OF CHEMICAL AND BIOLOGICAL TECHNIQUES FOR EVALUATION OF VAPORS IN AMBIENT AIR/MUTAGENICITY TESTING OF TWELVE (12) VAPOR-PHASE COMPOUNDS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/1-84/005 (NTIS PB84164219), 1984.
Description:
The overall objective of this research was to further validate Ames mutagenicity preincubation system for detection of vapors in ambient air. Parametes for detection of vapors in the Ames/Salmonella mutagenicity assay were investigated and 12 vapor-phase compounds were tested for mutagenic potential in the above validated protocol with strains TA97, TA98 and TA100, both with and without Aroclor-induced hamster and rat liver S9. The 12 vapor-phase compounds tested were: methylene chloride, formaldehyde, trichloroethylene, ethylene dichloride, epichlorohydrin, nitropropane, nitrobenzene, hydrogen sulfide, butene, propylene, acetylene, and ethylene oxide. Ethylene oxide, epichlorohydrin, ethylene dichloride and methylene chloride were mutagenic.