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ASSESSMENT OF IMMUNOTOXIC POTENTIAL OF THE FUNGICIDE DINOCAP IN MICE
Citation:
Smialowicz, R., R. Luebke, AND M. Riddle. ASSESSMENT OF IMMUNOTOXIC POTENTIAL OF THE FUNGICIDE DINOCAP IN MICE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/018 (NTIS PB93156230), 1992.
Description:
The immunotoxic potential of dinocap was evaluated in female C57BL/6J mice following in vivo and in vitro exposure to this fungicide. n in vivo studies, groups of mice were dosed by gavage with technical grade dinocap at dosages ranging from 12.5 to 50 mg/kg/d for seven or twelve days and selected immune functions examined. ice dosed at 50 mg/kg/d dinocap died after four days of dosing. Twelve days of dosing with dinocap at 25 mg/kg/d resulted in decreased thymus weights and cellularity, and increased spleen weights. Lymphoproliferative responses to concanavalin A (Con A) and phytohemagglutinin (PHA) were reduced in thymocytes from mice dosed at 25 mg/kg/d dinocap. he cytotoxic T lymphocyte (CTL) response to P815 mastocytoma cells was enhanced in mice exposed for 7 days to 25 mg/kg/d dinocap. xposure of mice for 7 days to 25 mg/kg/d dinocap also caused a significant reduction in the IgM and IgG plaque-forming cell (PFC) response to sheep red blood cells (SRBC). ime-course study indicated that dinocap-induced suppression of the IgM PFC response was due to a delay in the peak PFC response to SRBC. n vitro studies using murine thymocytes cultured with dinocap (10 ug/ml for 72 hr) resulted in suppression of the proliferative response to Con A and PHA. xposure of thymocytes to dinocap in vitro for as little as 30 minutes resulted in suppression of the mitogen-stimulated response in the absence of any apparent direct cytotoxic effect. hese results suggest that dinocap is immunotoxic in the mouse, causing effects on T lymphocytes.