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DIRECT DNA SEQUENCING OF PCR PRODUCTS
Citation:
Zimmerman, L. AND J.C. Fuscoe. DIRECT DNA SEQUENCING OF PCR PRODUCTS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/053 (NTIS PB92150671), 1991.
Description:
Experiments are described which elucidate some of the technical problems associated with the direct sequencing of polymerase chain reaction (PCR) amplified DNA. Sequencing primer purity, labeling methodology, and template preparation were explored. onditions are presented for the routine sequencing of single- and double-stranded PCR products.
Record Details:
Record Type:DOCUMENT( REPORT )
Product Published Date:12/31/1991
Record Last Revised:12/22/2005
Record ID:
32791