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IMMUNE ALTERATIONS IN RATS FOLLOWING SUBACUTE EXPOSURE TO TRIBUTYLTIN OXIDE
Citation:
Smialowicz, R., M. Riddle, R.R. Rogers, R. Luebke, C. Copeland, AND G. Ernst. IMMUNE ALTERATIONS IN RATS FOLLOWING SUBACUTE EXPOSURE TO TRIBUTYLTIN OXIDE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/331 (NTIS PB91149773), 1990.
Description:
Adult male Fischer 344 rats were dosed by oral gavage with bis(tri-n-butyltin) oxide (TBTO) in peanut oil on ten consecutive days, at dosages ranging from 1.25 to 15 mg/kg/day. ther groups of rats were dosed daily for 10 days by oral gavage with cyclophosphamide (CY) at dosages ranging from .75 to 6 mg/kg/day. hese rats served as positive controls for the immune assays employed. he immune function parameters examined included the following: elayed-type hypersensitivity (DTH) and antibody responses to bovine serum albumin (BSA), primary antibody responses to sheep red blood cells (SRBC) and trinitrophenyl lipopolysaccharide (TNP-LPA), and enumeration of splenic lymphocyte populations. he DTH and antibody responses to BSA were not effected by TBTO exposure; however these responses were suppressed in rats dosed with CY at 6 mg/kg/day. he plaque forming cell (PFC) response to the T cell-dependent antigen SRBC was enhanced in rats dosed with TBTO at from 5 to 15 mg/kg/day. n the other hand, the PFC response to the T cell-independent antigen TNP-LPS was unaffected by TBTO exposure. ats dosed with CY had suppressed PFC responses to SRBC and TNP-LPS at dosages of 3 and 6 mg/kg/day, respectively. numeration of splenic lymphocyte populations from TBTO-exposed rats revealed a reduction OX8- but not W3/25- or IgG-positive cells. hese results, as well as results from an earlier study from this lab, indicate that T lymphocytes are a primary target for TBTO-induced immune alterations and that the enhancement of the PFC response to SRBC in TBTO-exposed rats may be mediated by alterations in the suppressor (OX8-positive) T lymphocyte population.