Citation:

Ho, K.T., K. Mitchell, M. Zappala, AND R.M. Burgess. EFFECTS OF BRINE ADDITION ON EFFLUENT TOXICITY AND MARINE TOXICITY IDENTIFICATION EVALUATION (TIE) MANIPULATIONS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/236, 1995.

Description:

Little information is available concerning the effect of salinity adjustment on effluent storage and toxicity identification evaluation (TIE) performance. hese factors are important for accurate assessments of potential toxicity to marine organisms. he objective of this study was to determine (a) the effect of salinity adjustment (to 30 ppt), using hypersaline brine(100 ppt), on the toxicity of effluents stored up to 40 d, and (b) to determine the effect of salinity adjustment on TIE manipulations. hanges in effluent toxicity over time were examined by using a municipal and an industrial effluent. oxicity time series was performed for 16 d for the industrial effluent and 40 d for the municipal effluent. oxicity was measured with modified 48-h acutemysidopsis bahia and Menidia beryllina tests. esults indicate that, compared to day 0 test results, effluent stored with brine had fewer significant changes in toxicity than did effluent stored without brine. o determine the effects of brine addition on TIE manipulations, we conducted a series of manipulations in which one aliquot of an effluent had brine added prior to the TIE manipulations and the other aliquot had brine added after the TIE manipulation. he manipulations conducted were EDTA addition, sodium thiosulfate addition, C18, extraction, aeration, filtration, and graduated pH manipulations. oxicity was measured with the modified 48-h acute mysid test. ddition of brine had no effect on the outcome of TIE manipulations. e have concluded that it is operationally easier to add brine as soon as possible after sampling and that effluent tests should be conducted as soon as practical.

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