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SURVIVAL AND DEGRADATIVE CAPACITY OF PSEUDOMONAS PUTIDA INDUCED OR CONSTITUTIVELY EXPRESSING PLASMID-MEDIATED DEGRADATION OF 2,4-DICHLOROPHENOXYACETATE (TFD) IN SOIL
Citation:
Short, K., R. Seidler, AND R. Olsen. SURVIVAL AND DEGRADATIVE CAPACITY OF PSEUDOMONAS PUTIDA INDUCED OR CONSTITUTIVELY EXPRESSING PLASMID-MEDIATED DEGRADATION OF 2,4-DICHLOROPHENOXYACETATE (TFD) IN SOIL. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/511 (NTIS PB91182196), 1990.
Description:
Survival of genetically altered Pseudonomas putida strains harboring an inducible plasmid, pRO101, or a constitutive plasmid, pRO103, was compared. hese plasmids encoded for the degradation of 2,4-dichlorophenoxyacetate (TFD) to 2-chloromaleylacetate, and the maintenance of either plasmid did not alter survival of P. putida PPO301 (pRO101) or PPO301 (pR) 103) in an unamended agricultural soil. n a parallel study, aphanus sativus (radish) seeds failed to germinate in uninoculated and PPO301-inoculated soil amended with 500 ppm TFD. eed germination was 53 and 80% in soils inoculated with PPO301 (pRO101) and PPO301 (prO103), respectively (P<0.001). owever, the difference in the rate of TFD degradation between the native soil and soil inoculated with plasmid-bearing P. putida was probably related to the relatively high inoculum density of P. putida strains (10 8 cfu) and the relatively low population density of TFD metabolizers indigenous to the soil.