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REGIOSPECIFIC DECHLORINATION OF PENTACHLOROPHENOL BY DICHLOROPHENOL-ADAPTED MICROORGANISMS IN FRESHWATER, ANAEROBIC SEDIMENT SLURRIES
Citation:
Bryant, F., D. Hale, AND J. Rogers. REGIOSPECIFIC DECHLORINATION OF PENTACHLOROPHENOL BY DICHLOROPHENOL-ADAPTED MICROORGANISMS IN FRESHWATER, ANAEROBIC SEDIMENT SLURRIES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/221 (NTIS PB92101674), 1991.
Description:
The reductive dechlorination of pentachlorophenol (PCP) was investigated in anaerobic sediments that contained nonadapted or 2,4- or 3,4-dichlorophenol-adapted microbial communities. daptation of sediment communities increased the rate of conversion of 2,4- or 3,4-DCP to monochlorophenols (CPs) and eliminated the lag phase before dechlorination was observed. oth 2,4- and 3,4-DCP-adapted sediment communities dechlorinated the six DCP isomers to CPs. he specificity of chlorine removal from the DCP isomers indicated a preference for ortho-chlorine removal by 2, 4-DCP-adapted sediment communities and for a-chlorine removal by 3,4-DCP-adapted sediment communities. ediment slurries containing nonadapted microbial communities either did not dechlorinate PCP or did so following a lag phase of at least 40 days. ediment communities adapted to dechlorinate 2,4- or 3,4-DCP dechlorinated PCP without PCP without an initial lag phase. he 2,4-DCP-adapted communities initially removed the ortho-chlorine from PCP, whereas the 3,4-DCP-adapted communities initially removed the a-chlorine from PCP. :1 mixture of the adapted sediment communities also dechlorinated PCP without a lag phase. ntermediate products of degradation--2,3,5,6-tetrachlorophenol, 2,3,5-trichlorophenol, 3,5-DCP, 3-CF, and phenol--were identified by a combination of cochromatography (high-pressure liquid chromatography) with standards and gas chromatography-mass spectrometry.
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REGIOSPECIFIC DECHLORINATION OF PENTACHLOROPHENOL BY DICHLOROPHENOL-ADAPTED MICROORGANISMS IN FRESHWATER, ANAEROBIC SEDIMENT SLURRIES