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SPECIES AND STRAIN COMPARISONS OF IMMUNOSUPPRESION BY 2-METHOXYETHANOL AND 2-METHOXYACETIC ACID
Citation:
Smialowicz, R., M. Riddle, AND W. Williams. SPECIES AND STRAIN COMPARISONS OF IMMUNOSUPPRESION BY 2-METHOXYETHANOL AND 2-METHOXYACETIC ACID. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/514 (NTIS PB95148763), 1994.
Description:
2-Methoxyethanol (ME) and its principal metabolite 2-methoxyacetic acid (MAA) have been shown in our laboratory to be immunosuppressive in male Fischer 344 rats. n this study several strains of 12 week old female rats and mice were used to compare the immunosuppressive activity of equimolar concentrations of ME and MAP. on the trinitrophenyl-lipopolysaccharide (TNP-LPS) antibody plaque-forming cell (PFC) response, which we previously demonstrated to be a sensitive end point. emale inbred Lewis, Fischer 344 and Wistar/Furth, and outbred Sprague-Dawley rats were dosed by gavage with either ME or MAA at dosages of 0.33 to 2.64 mmol/kg/d for 10 consecutive days. emale inbred C3H and C57BL/6J, hybrid B6C3F1, and outbred CD-1 mice were similarly dosed with equimolar dosages of 0.66 to 5.28 mmol/kg/d ME or MAA. ll animals were immunized on day 9 of dosing and PFC responses evaluated 3 days later. uppression of the PFC response was observed in a@ ' strains of rats at 2.64 mmol/kg/d ME or MAA. ewis and Wistar/Furth rats- were found to be the most sensitive strains with suppression at levels as low as 0.66 mmol/kg/d ME or MAA. hile ME and MAA dosing resulted in suppression of the TNP PFC response i-n all the rat strains tested, such treatment did not suppress this PFC response in any of the mouse strains examined. hese results-indicate that under the conditions of this study rats, but not mice, are immunosuppressed by ME and MAA exposure, and that the susceptibility to immunosuppression differsamong rat strains.