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MUTAGENICITY OF BENZO(A)PYRENE METABOLITES GENERATED ON THE ISOLATED PERFUSED LUNG FOLLOWING PARTICULATE EXPOSURE (JOURNAL VERSION)
Citation:
Schoeny, R. AND D. Warshawsky. MUTAGENICITY OF BENZO(A)PYRENE METABOLITES GENERATED ON THE ISOLATED PERFUSED LUNG FOLLOWING PARTICULATE EXPOSURE (JOURNAL VERSION). U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-83/030 (NTIS PB83244087), 1983.
Description:
The isolated perfused rabbit lung (IPL) is being used to study the effects of particulate exposure on the pulmonary metabolism of benzo(a)pyrene (BaP). Pasturealla-free New Zealand white rabbits were treated intraperitoneally with BaP prior to kill. The isolated lungs were then administered either (14)C-labeled BaP alone or BaP plus Fe2O3 or fly ash by intratracheal injection. Rates of appearance of BaP metabolites in the perfusing blood were determined. The extent of metabolism, distribution of metabolites, and types of metabolites produced were quantified for various lung tissue types by high-performance liquid chromatography and liquid scintillation spectrometry.