You are here:
ONCOGENE ALTERNATIONS IN IN VITRO TRANSFORMED RAT TRACHEAL EPITHELIAL CELLS
Citation:
Mass, M., N. Schorschinsky, J. Lassley, AND D. Beerman. ONCOGENE ALTERNATIONS IN IN VITRO TRANSFORMED RAT TRACHEAL EPITHELIAL CELLS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/083 (NTIS PB90245432), 1990.
Description:
Ten derivations of rat tracheal epithelial (RTE) cells, including normal cells, normal primary cultures, 7 tumorigenic cell lines and 1 non-tumorigenic cell line transformed by treatment with 7,12-dimethylbenz(a)anthracene (DMBA), benzo(a)pyrene (BP) and/or 12-0-tetradecanoylphorbol-l3-acetate (TPA) were examined for oncogene alterations. hese analyses included amplification, restriction length polymorphisms (RFLPs), and changes in the expression of RNA transcripts of Ha-ras, Ki-ras, and c-myc. wo MBA-derived cell lines displayed RFLPs after Eco RI digestion and probing for Ki-ras. o abnormalities of Ha-ras were seen after digestion with Bam HI, Eco RI, or Hind III. o extra Pst I (Ha-ras codon 12, GGA to CGA) or Xba I (Ha-ras codon 61, CAA to CTA) sites were seen after probing for Ha-ras. n one cell line derived by DMBA-treatment, changes in the c-myc restriction pattern were seen after digestion with Barn HI and Hind III. nalysis of CCGG sequences by Hpa Il/Msp I digestion indicated that was highly methylated, exhibited limited methylation, and CCGG sequences were not methylated. n addition, normal cells removed from tracheas and those in primary cultures displayed Hpa II digestion patterns that were distinguishable from those of transformed cell lines by the presence of an apparent extra methylation site. NA dot-blots showed no consistent differences between normal and transformed cells when probed with or expression of the most highly expression could not be detected. hese studies show a number of different changes in oncogenes Ha-ras, Ki-ras, and c-myc, but they do not implicate any single oncogene in cell transformation in vitro.