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SUBACUTE AND SUBCHRONIC TOXICITY OF ETHYLENE GLYCOL ADMINISTERED IN DRINKING WATER TO SPRAGUE-DAWLEY RATS
Citation:
Robinson, M., C. Pond, R. Laurie, J. Bercz, G. Hennings, AND L. Condie. SUBACUTE AND SUBCHRONIC TOXICITY OF ETHYLENE GLYCOL ADMINISTERED IN DRINKING WATER TO SPRAGUE-DAWLEY RATS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/090 (NTIS PB90245374), 1990.
Description:
Subacute (10-day) and subchronic (90-day) toxicity studies of ethylene glycol (EG) were conducted in male and female sprague-Dawley rats to provide the U.S. Environmental Protection Agency's (EPA) Office of Drinking Water with toxicity data for final preparation of a Health Advisory for the chemical. thylene glycol was administered in drinking water at concentrations of 0.5, 1.0, 2.0, and 4.0% for both sexes in the 10-day study. ased on a projected consumption rate of 100 ml/kg/day, the respective doses on a mg/kg/day basis would be 554, 1108, 2216, and 4432. hese dose levels were also used in the 90-day study for females, but dose levels for the males in the 90-day study were 0.25, 0.5, 1.0, and 2.0% (227, 554, 1108, and 2216 mg/kg/day). t time of sacrifice necropsies were performed an tissues were prepared for histoligical evaluation. lood samples were taken for hematology and clinical chemistry determinations. ody weights were measured weekly. ater and food consumption were determined three times weekly. o mortality occurred in the 10-day study. n the 90-day study 8/10 females and 2/10 males in the high dose group died prior to sacrifice. ody weights were suppressed in a dose response fashion for males and females. emoglobin, hematocrit, erythrocytes, and leukocytes were all significantly decreased in female rats receiving 4% EG for 10 days. he most significant histopathological findings, seen predominantly in males, were kidney lesions which included calcium oxalate crystals in tubules and pelvic epithelium; tubular dilation and degeneration; intratubular proteinaceous material; and inflammation in tubules and pelvic epithelium. t the same dose of ethylene glycol, males had more kidney lesions and much higher incidence and severity of lesions than the females.