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COMPARISON OF ANTIOXIDANT SUBSTANCES IN BRONCHOALVEOLAR LAVAGE CELLS AND FLUID FROM HUMANS, GUINEA PIGS, AND RATS
Citation:
Slade, R., K. Crissman, J. Norwood, H. Koren, AND G. Hatch. COMPARISON OF ANTIOXIDANT SUBSTANCES IN BRONCHOALVEOLAR LAVAGE CELLS AND FLUID FROM HUMANS, GUINEA PIGS, AND RATS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/020 (NTIS PB94137346), 1993.
Description:
Antioxidants located in the lining layer of the bronchoalveolar region of the respiratory tract may be important in determining sensitivity of lung tissues to inhaled pollutants. he present study addressed the question of whether there are species differences in the levels of some of these antioxidants in bronchoalveolar lavage (BAL) fluid. easurements were made of ascorbic acid (AH2), glutathione (GSH), uric acid (UA), a-tocopherol (AT), total protein and lipid phosphorus (lipid P) in bronchoalveolar lavage (BAL) of humans, guinea pigs (both AH2- deficient and supplemented), and rats ("young" rats: 70 days old and "old" rats: 20 mo old). n the BAL cell-free fraction, humans had 10 to 100 times more AT/lipid P than the other species. oung rats had the highest AH2/Protein (200 nmol/mg) ratio in the cell-free fraction, 3 times as great as old rats and 7 to 50 times as great as the other species. n BAL cells AT was present at much greater concentrations than in the cell-free fraction, and there appeared to be an inverse relationship between AT in the cells and the cell-free fraction. umans had the lowest cellular levels of GSH (2.9 nmol/mg protein) and AH2 (3.5 nmol/mg protein). oung rats had 2.5 times as much AH2 (50 nmol/mg protein) in cells as old rats and 3 to 15 times as great as ,guinea pigs and humans. hese data demonstrate that there are large differences in the concentrations of lavageable antioxidants, not only between species but also between the cellular and extracellular fractions.