You are here:
IN VITRO ALUMINUM INHIBITION OF BRAIN PHOSPHOINOSITIDE METABOLISM:COMPARISON OF NEONATAL AND ADULTS RATS
Citation:
Mundy, W., T. Shafer, T. Freudenrich, AND A. Nostrandt. IN VITRO ALUMINUM INHIBITION OF BRAIN PHOSPHOINOSITIDE METABOLISM:COMPARISON OF NEONATAL AND ADULTS RATS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/351, 1995.
Description:
Recent evidence indicates that the neurotoxic metal aluminum interferes with the phosphoinositide second messenger system in adult rats both in vitro and in vivo. e have examined the age-related effects of aluminum chloride (AlCl3) on receptor-stimulated inositol phosphate (IP) accumulation in brain slices from neonatal and adult rats in vitro. arbachol-stimulated (1 mM) IP accumulation was greatest in frontal cortex slices from 7 day old rats, decreased in 14 day old and 21 day old rats, and was lowest in adults (120 days old). lCl3 (500 uM) inhibited both basal and carbachol-stimulated IP accumulation in neonatal and adult rats. he effects of AlCl3 were concentration-related and produced significant decreases (15-25%) in IP accumulation at 500 and 1000 uM. he concentration-response curve for AlCl, was similar in 7 day old and adult rats. lCl3 reduced carbachol-, norepinephrine- and quisqualate-stimulated IP accumulation in both 7 day old and adult rats. he effects of 500 uM AlCl3 were slices prepared from frontal cortex, accumulation was greater in slices issue, AlCl3 (500 uM) decreased IP both ages. luminum produced C in cortical homogenates which was show that in vitro exposure to aluminum decreases IP accumulation through a mechanism which is not age-dependent examined on carbachol-stimulated IP accumulation in hippocampus, striatum, and cerebellum. lthough IP from the 7 day old rats compared to adults in each accumulation by approximately 20% in all regions at concentration-dependent inhibition of phospholipase is similar in 7 day old and adult rats. hese results show that in vitro exposure to aluminum decreases IP accumulation through a mechanism which is not age-dependent.