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ALTERED STEROIDOGENESIS IN WHOLE-OVARY AND ADRENAL CULTURE IN CYCLING RATS
Citation:
Berman, E. AND J. Laskey. ALTERED STEROIDOGENESIS IN WHOLE-OVARY AND ADRENAL CULTURE IN CYCLING RATS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/046 (NTIS PB94137080).
Description:
Cultures of minced, whole-ovary (whole-ovary culture) were used to determine if three selected chemicals altered steroidogenic profiles. irst, phenolaulfonthalein (PST), when used in culture medium, was tested for its influence on in vitro steroidogenesis. ext, aminoglutethimide (AGTP; 0 or 150 mg/kg once) and di(2-ethylhexyl)phthalate (DEHP; 0 or 1500 mg/kg/10d) were administered in vivo to young adult cycling rats, and the ovaries and adrenals removed and cultured for 1 hr. varian steroidogenic profiles of progesterone (P), testosterone (T), and estradiol (E) released into the medium were measured using radioimmunoassay techniques. ST in medium significantly decreased ovarian P-production and altered T and E production so that the T/E ratio was significantly altered. herefore, it was excluded in the subsequent studies. EHP altered steroid profiles in such a way that proestrus appeared to be delayed. GTP decreased P and E production significantly and T production was increased slightly in proestrus ovaries. hese AGTP alterations in T and E resulted in a highly significant increase in the T/E ratio. drenals from the DEHP and AGTP experiments were also cultured for I hr and P assayed in the medium. GTP, but not DEHP, significantly increased the production of P in adrenals. hole-ovary culture is recommended as an in vitro test for chemicals suspected of interfering with steroidogenesis in vivo. his test model should be placed strategically between in vivo studies of reproductive toxicity and complex in vitro mechanistic studies.
