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BIOLOGICAL MONITORING SYSTEMS FOR HAZARDOUS WASTE SITES (PRODUCTION AND ANALYSIS OF ANALYTICAL REFERENCE MATERIALS)
Citation:
Bohman, V., C. Blincoe, G. Miller, R. School, W. Sutton, AND L. Williams. BIOLOGICAL MONITORING SYSTEMS FOR HAZARDOUS WASTE SITES (PRODUCTION AND ANALYSIS OF ANALYTICAL REFERENCE MATERIALS). U.S. Environmental Protection Agency, Washington, D.C., EPA/600/4-89/007.
Description:
EPA programs in pesticides, toxics, and hazardous waste require analytical reference materials. This project emphasized the collection of and analysis of urine, fat, and blood for ultimate use as reference samples. Another objective of the project concerned the practicality of using certain metabolites to indicate previous exposure to chlorinated hydrocarbons. The reference samples of urine, fat, etc., can, with verified compound concentrations, be used as qualifying samples when evaluating a technique to use for a particular analysis. However, the reference materials may be of greatest benefit when used by laboratories to determine analytical accuracy for samples of human urine, blood, etc. This is because the standards, like the unknown samples, will contain pollutant compounds and associated metabolites (all in vivo incorporated). Dairy animals were used during this study primarily because substantial quantities of tissue and body fluid could be collected from the previously dosed cattle. Each animal was given daily oral doses of selected pollutant chemicals so that the urine, blood, etc., would contain detectable concentrations of the dosing compound(s) and some of the associated major metabolites. Aliquots of the respective large colume samples were chemically analyzed and selected as potential reference material. Some of the samples provided by this study have alread been used by an ongoing EMSL-LV progam for analytical method optimization.