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Strategies for integrating transcriptional profiling into high throughput toxicity testing (SOT Symposium Workshop presentation)
Citation:
Thomas, R. Strategies for integrating transcriptional profiling into high throughput toxicity testing (SOT Symposium Workshop presentation). Presented at SOT annual meeting, San Diego, CA, March 22 - 26, 2015. https://doi.org/10.23645/epacomptox.5083444
Impact/Purpose:
SOT symposium workshop presentation
Description:
Presentation Description: The release of the National Research Council’s Report “Toxicity Testing in the 21st Century: A Vision and a Strategy” in 2007 initiated a broad-based movement in the toxicology community to re-think how toxicity testing and risk assessment are performed. ToxCast and the associated Tox21 effort has screened thousands of chemicals across hundreds of high-throughput in vitro screening assays in concentrations-response format to identify potential biological targets and the concentrations at which they are affected. Although this paradigm has been useful for evaluating chemical effects, there is recognition that, as a whole, the suite of ToxCast and Tox21 assays cover a limited slice of biological space. New higher-throughput transcriptomic technologies have been developed that could be used to cover a broader slice of biological space for less cost. These technologies could be deployed in a tiered fashion to complement the existing suite of high-throughput in vitro screening assays; however, number of challenges must be addressed prior to using these technologies. The challenges include what in vitro systems to use provide the greatest representation of potential biological targets for chemicals, what platform is most appropriate for this application, what subset of genes should be measured, and how best to interpret the profiles. A strategy for integrating these new transcriptomic technologies into high throughput toxicity testing will be presented and the challenges discussed. (20 min)
URLs/Downloads:
DOI: Strategies for integrating transcriptional profiling into high throughput toxicity testing (SOT Symposium Workshop presentation)
HIGHTHROUGHPUTTRANSCRIPTOMICS_SOT_MARCH_2015_V3.PDF (PDF, NA pp, 2980.246 KB, about PDF)