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DNA ADDUCTS AND PERSONAL AIR MONITORING OF CARCINOGENIC POLYCYCLIC AROMATIC HYDROCARBONS IN AN ENVIRONMENTALLY EXPOSED POPULATION
Citation:
Binkova, B., J. Lewtas, I. Miskoca, J. Lenicek, AND R. Sram. DNA ADDUCTS AND PERSONAL AIR MONITORING OF CARCINOGENIC POLYCYCLIC AROMATIC HYDROCARBONS IN AN ENVIRONMENTALLY EXPOSED POPULATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/523.
Description:
The effect of personal exposure to air pollution on DNA adducts in humans was analyzed in a group (N=30) of women working outdoors (up to 8 h/day) as postal workers or gardeners in the city of Teplice, CZ (Northern Bohemia) where winter. inversions may result in high levels of air pollution from coal combustion. en of these women were followed up during the next winter season by repeated personal exposure monitoring and analysis of the DNA adducts in their white blood cells (in 4 sampling periods). ersonal exposure monitoring for respirable particles (<2.5um) was conducted for the 24 h period prior collection of blood and urine. article extracts were analyzed for carcinogenic PAH. rine samples were collected for cotinine analysis to control for exposure to tobacco smoke. NA isolated from white blood cells was analyzed by 32P-postlabeling with butanol enrichment procedure. here were 21 nonsmokers and 9 light smokers in the pilot study and only nonsmokers in the follow-up study. n the both studies high personal exposure variability between the individuals sampled the same day was observed. e found a significant correlation (r=0.541, p<0.016) between individual exposure to carcinogenic PAH and DNA adducts for the group of nonsmokers (N=21). o significant difference in DNA adduct levels was found between nonsmokers and smokers. n the follow-up study, during only 1 sampling period, the ambient and personal air monitors exhibited a significantly elevated exposure to respirable particles and carcinogenic PAH. he follow-up study showed a significant effect of personal exposure on DNA adduct levels and their relationship with the short-term exposure to carcinogenic PAH. he results suggest that DNA levels in white blood cells reflect a short-term environmental exposure.