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Part 2: Sensitivity comparisons of the insect Centroptilum triangulifer to Ceriodaphnia dubia and Daphnia magna using standard reference toxicants; NaCl, KCl and CuSO4
Citation:
Struewing, K., P. Weaver, Jim Lazorchak, B. Johnson, D. Funk, AND D. Buckwalter. Part 2: Sensitivity comparisons of the insect Centroptilum triangulifer to Ceriodaphnia dubia and Daphnia magna using standard reference toxicants; NaCl, KCl and CuSO4. CHEMOSPHERE. Elsevier Science Ltd, New York, NY, 139(11):597-603, (2015).
Impact/Purpose:
Publication covers the sensitivity of and presents new test methods for a parthenogenic mayfly, Centriptilum triangulifer
Description:
Criteria for establishing water quality standards that are protective of all native biota are generally based upon laboratory toxicity tests. These test utilize common model organisms that have established test methods. However, only a small portion of species have established methods so they should be the most sensitive or inclusive of all taxa. In order to examine a potential under-representation in emerging aquatic invertebrates the US Environmental Protection Agency has cultured a parthenogenetic invertebrate, the insect Centroptilum triangulifer, which potentially has higher sensitivity to certain toxicants. This study established a 48 hr. acute and a 14 day chronic testing procedure for C. triangulifer and compared its sensitivity to two model invertebrates, Ceriodaphnia dubia and Daphnia magna. Toxicity tests were conducted to determine mortality and growth effects using standard reference toxicants: NaCl, KCl and CuSO4. In 48 hr. acute tests, the average LC50 for the mayfly was 659 mg/L NaCl, 1957 mg/L KCl, and 11 µg/L CuSO4. IC25 values, using weight as the endpoint, were 228 mg/L NaCl, 356 mg/L KCl and 5 µg/L CuSO4. In tests with C. triangulifer, the weight endpoint was more sensitive to the toxicants than body length and head capsule width. C. triangulifer was the most sensitive species in NaCl acute and chronic growth tests. KCl at concentrations tested for the two daphnid species failed to produce mortality in C. triangulifer bioassays, but the species was equally or more sensitive than C. dubia and D. magna for growth measurements. C. triangulifer was the most sensitive species during acute testing and for growth parameters in chronic tests. This study determined C. triangulifer has great potential and benefits for use in ecotoxicological studies.
