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Method 447.0 - Determination of Chlorophylls a and b and Identification of Other Pigments of Interest in Marine and Freshwater Algae Using High Performance Liquid Chromatography with Visible Wavelength Detection
Citation:
Arar, E. J. Method 447.0 - Determination of Chlorophylls a and b and Identification of Other Pigments of Interest in Marine and Freshwater Algae Using High Performance Liquid Chromatography with Visible Wavelength Detection. U.S. Environmental Protection Agency, Washington, DC, 1997.
Impact/Purpose:
see description
Description:
This method provides a procedure for determination of chlorophylls a (chl a) and b (chl b) found in marine and freshwater phytoplankton. Reversed phase high performance liquid chromatography (HPLC) with detection at 440 nm is used to separate the pigments from a complex pigment mixture and measure them in the sub-microgram range. For additional reference, other taxonomically important yet commercially unavailable pigments of interest are identified by retention time.