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INFLUENCE OF ANTIBIOTICS ON THE INTESTINAL TRACT SURVIVAL AND TRANSLOCATION OF ENVIRONMENTAL PSEUDOMONAS SPECIES
Citation:
George, S., M. Kohan, D. Whitemore, J. Creason, AND L. Claxton. INFLUENCE OF ANTIBIOTICS ON THE INTESTINAL TRACT SURVIVAL AND TRANSLOCATION OF ENVIRONMENTAL PSEUDOMONAS SPECIES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/164 (NTIS PB91115865).
Description:
The environmental release of microorganisms has prompted the investigation of potential health effects associated with their release. n this study, survival and translocation to the spleen and liver of several environmental Pseudomonas spp. was investigated in antibiotic-treated mice. . aeruginosa strain BC16 and P. maltophilia strain BC6, isolated from a commercial product for PCB degradation, P. aeruginosa strain AC869, a 3,5-dichlorobenzoate degrader, and P. cepacia strain AC1100, an organism that metabolizes 2,4,5-trichlorophenoxyacetic acid were examined for their survival capabilities in the intestines of mice dosed with clindamycin, ianamycin, rifampicin, or spectinomycin. ouse intestinal isolate, strain PAMG, was included in the study. ollowing antibiotic pretreatment (1 mg twice daily for 3 days), mice were dosed by gavage with 10 9 colony forming units (CFU) of the individual Pseudomonas spp. y the end of the 5 day test period, strains AC869 and PAMG survived in kanamycin-, rifampicin-, spectinomyicn-, and clindamycin-treated animals. tatistically significant (p 0.05) increase in survival of strain AC869 and PAMG was observed in clindamycin-treated mice for the test period. ifampicin increased survival of strain AC869 and spectinomycin was selective for strain. train BC6, which is resistant to rifampicin, was detected in the intestinal tract 5 days after dosing. train BC16, a clindamycin resistant strain, was detected in clindamyin-treated mice and the untreated control animals. ranslocation to the spleen as observed in spectinomycin- and clindamycin-treated mice.