You are here:
Acute Neurobehavorial Toxicity of Flame Retardant Replacement Compounds in Zebrafish Larvae
Citation:
Shaffer, R., K. Jarema, D. Hunter, AND S. Padilla. Acute Neurobehavorial Toxicity of Flame Retardant Replacement Compounds in Zebrafish Larvae. SETAC, RALEIGH, NC, March 13, 2015.
Impact/Purpose:
To be presented at the SETAC LOCAL MTG.
Description:
As polybrominated diphenyl ethers (PBDEs) are phased out, numerous compounds areemerging as potential replacement flame retardants for use in consumer and electronicproducts. Little is known, however, about the neurobehavioral toxicity of thesereplacements. This study evaluated the acute neurobehavioral effects of t-butylphenyldiphenyl phosphate (BPDP), 2-ethylhexyl diphenyl phosphate (EHDP), isodecyl diphenylphosphate (IDDP), isopropylated phenyl phosphate (IPP), tricresyl phosphate (TMPP),triphenyl phosphate (TPHP), tetrabromobisphenol A (TBBP A), tris (2-chloroethyl)phosphate (TCEP), tris (1,3-dichloroisopropyl) phosphate (TDCIPP), tri-a-cresylphosphate (TOCP), and 2,2-,4,4'-tetrabromodiphenyl ether (BDE-47) in zebrafish (Daniarerio) larvae. Larvae were exposed to test compounds (dose-response; varying dosesbetween 0.4- 120 !J.M; n:::::24 larvae per dose per compound) at 6 days post fertilization, and locomotor activity was assessed using automated video tracking in 96-well microtiter plates during three sequential dark/light/dark test periods beginning 30 minutes after chemical exposure. All compounds produced changes in activity, usually manifested as hypoactivity in the dark periods and hyperactivity in the light periods. Across all doses and time periods, PBDE-47 and TCEP produced the weakest acute effects, while EHDP,IPP, EPHP, and TBBP A produced the strongest effects. These data indicate that acute exposure to selected flame retardant replacement candidates alters swimming behavior in young zebrafish. This abstract may not necessarily reflect official Agency policy.