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An Animal Model of Active (Act) Versus Sedentary (Sed) Lifestyle and Susceptibility to Air Pollution: Response to Ozone (O3) in Female Sprague-Dawley Rats Allowed to Train Chronically On Running Wheels
Citation:
Gordon, C., P. Phillips, A. Johnstone, K. Jarema, T. Beasley, A. Ledbetter, J. Lehmann, C. Aydin, M. Schladweiler, AND U. Kodavanti. An Animal Model of Active (Act) Versus Sedentary (Sed) Lifestyle and Susceptibility to Air Pollution: Response to Ozone (O3) in Female Sprague-Dawley Rats Allowed to Train Chronically On Running Wheels. Society of Toxicology Annual Meeting, San Diego, CA, March 23 - 27, 2015.
Impact/Purpose:
This abstract will be presented at the Society of Toxicology Annual Meeting, March 23-27, 2015, San Diego, CA Dr. Gordon advised entering RAP code of SHC 2.2.1.2
Description:
Epidemiological data suggest that a sedentary lifestyle may contribute to increased suseptibility to environmental pollutants. Furthermore, the association between a sedentary pattern and development of obesity may exacerbate susceptibility. To study the effects of ACT vs. SED living on susceptibility to air pollution, we developed an animal model by providing 60 d old rats with continuous access to running wheels. Wheel activity (WA) was monitored continuously. SED rats were housed in standard cages without wheels. After training for 12 wk, rats were exposed to 03 (0, 0.25,0.5,or 1.0 ppm) for 5 hr/d,1 d/wk, for 6 wk (N=10 per group). Body composition was measured (Bruker) periodically. Respiratory parameters (tidal volume, minute ventilation, frequency, and enhanced pause (penH)) were assessed (Buxco) prior to 03 and 24 hr after the 5th 03 exposure. After 12 wk of training, ACT rats lost 2% body fat and had significant reductions in penH, minute ventilation, and frequency. Peak WA was reduced by 40% during the night after exposure to 1.0 ppm 03. After the 5th 03 exposure, body weight (BW) and %fat were reduced in SED but not ACT rats. PenH was significantly elevated in SED but not ACT rats exposed to 1.0 ppm 03. Overall, chronic exercise training appears to protect rats from respiratory impairment when measured 24 hr after 03 exposure. 03-induced reductions in body fat and BW loss are also ameliorated with chronic exercise. A SED lifestyle may increase pulmonary and metabolic susceptibility to 03. This is an abstract of a proposed presentation and does not reflect US EPA policy.