You are here:
Impact of diet on ozone-induced pulmonary and systemic effects in female Brown Norway (BN) rats
Citation:
Bass, V., M. Schladweiler, S. Snow, C. Gordon, K. Jarema, P. Phillips, A. Ledbetter, D. Miller, J. Richards, AND U. Kodavanti. Impact of diet on ozone-induced pulmonary and systemic effects in female Brown Norway (BN) rats. Society of Toxicology Annual Meeting, San Diego, CA, March 22 - 26, 2015.
Impact/Purpose:
Recent studies have shown a link between air pollution and increasing rates of metabolic syndrome. Dietary factors are proposed to play a contributing role in the development of air pollution-induced metabolic impairments. These data suggest that high fat and high fructose diets had a small influence on pulmonary and systemic metabolic effects of O3.
Description:
Impact of diet on ozone-induced pulmonary and systemic effects in female Brown Norway (BN) ratsV.L. Bass1, M.C. Schladweiler2, S. Snow5, C.J. Gordon4, K.A. Jarema4, P. Phillips4, A.D. Ledbetter2, D.B. Miller3, J.E. Richards2, U.P. Kodavanti2. 1. SPH, UNC, Chapel Hill2. EPHD, NHEERL, USEPA, Research Triangle Park, NC3. CIT UNC, Chapel Hill4. TAD, NHEERL, USEPA, Research Triangle Park, NC 5. ORISE, Research Triangle Park, NCRecent studies have shown a link between air pollution and increasing rates of metabolic syndrome. Dietary factors are proposed to play a contributing role in the development of air pollution-induced metabolic impairments. Recently, ozone (O3) exposure was shown to induce acute systemic metabolic changes in male rats, with effects modified by diet. In this study, we hypothesized that female Brown Norway (BN) rats would respond similarly to a 3 month high fructose or high fat dietary regime, with induction of metabolic alterations and obesity, as well as O3-induced modification of pulmonary injury and systemic metabolic effects. Female BN rats began dietary regimes at 1 month of age (normal, high fructose, or high fat chow). At 4 months of age, exposure began to air or O3 (0.8 ppm), 5 hr/day, 1 day/week, for either 1 week or 4 consecutive weeks. Body composition and glucose tolerance were monitored during the dietary regime and immediately following weekly O3 exposures. Pulmonary toxicity was also assessed immediately after the 1st and 4th week of exposure (n=10). Neither high fat nor high fructose diet increased body weight but leptin was higher in rats on high fat diet. No glucose intolerance was noted by diet alone. An increase in circulating triglycerides was seen in rats on high fructose but not high fat diet. A significant glucose intolerance was observed following first O3 exposure in rats on normal and high fructose diets, and after 3rd week exposure in rats on the high fat diet. All dietary groups had elevated serum leptin in response to a single O3 exposure. O3-induced lung injury was not impacted by any dietary regimen in female BN rats. These data suggest that high fat and high fructose diets had small influence on pulmonary and systemic metabolic effects of O3. (Does not reflect US EPA policy.)