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GAMMA-GLUTAMYL TRANSFERASE (GGT) ACTIVITY AND BIOCHEMICAL CHARACTERIZATION OF RAT VISCERAL YOLK-SAC DURING GESTATION WITH OR WITHOUT TRYPAN BLUE EXPOSURE
Citation:
Andrews, J., M. Ebron-Mccoy, T. Logsdon, AND J. Rogers. GAMMA-GLUTAMYL TRANSFERASE (GGT) ACTIVITY AND BIOCHEMICAL CHARACTERIZATION OF RAT VISCERAL YOLK-SAC DURING GESTATION WITH OR WITHOUT TRYPAN BLUE EXPOSURE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/081.
Description:
Yolk-sacs from untreated Sprague Dawley rat conceptuses were removed on days 9-18 of gestation and examined for gamma-glutamyl transferase (GGT), alkaline phosphatase (AP), lactate dehydrogenase (LDH) and glutamic-oxaloacetic transaminase (GOT) activities. All enzyme activities were found to increase through gestation in total activity as well as in specific activity. Protein (PRO) and urea nitrogen (UN) were also found to increase through gestation whereas triglyceride (TRI) increased steadily from day 9-13 and then appeared to plateau through day 18 of gestation. Additional rats were treated on day 8 of gestation with 75 mg trypan blue/kg body weight and yolk sacs taken on days 9-18 of gestation. Yolk sac GGT and GOT activities from trypan blue (TB) treated rats were significantly higher than the respective controls during early gestation but recovered to or were lower than control levels during midgestation. GGT activity in treated rats was significantly lower than controls on days 17-18 of gestation. Thus, the yolk sac enzymes monitored which are associates with nutrition and normal growth increased in activity through day 18 of gestation. The yolk sac toxicant, trypan blue, significantly affected enzymatic activity of these enzymes at various time periods during gestation and did result in significant changes in yolk sac protein and triglyceride content.